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![ELISA: TIM-1/KIM-1/HAVCR Antibody (3B3) [NBP2-81119] - ELISA of anti-TIM-1 antibody on TIM-1-Fc fusion protein. Binding curves of the rabbit chimeric version of the anti-TIM-1 antibody 3B3 (NBP2-81119; red line) and isotype control (NBP2-52638, anti-Fluorescein; blue line) to an ELISA plate coated with mouse TIM-1-Fc fusion protein at a concentration of 5 ug/ml. A 3-fold serial dilution from 10000 to 0.17 ng/ml was performed using NBP2-81119. For signal detection, a 1:4000 dilution of anti-rabbit IgG1 HRP antibody was used.](http://images.novusbio.com/fullsize/TIM-1-KIM-1-HAVCR-Antibody-3B3-ELISA-NBP2-81119-img0001.jpg "ELISA: TIM-1/KIM-1/HAVCR Antibody (3B3) [NBP2-81119] - ELISA of anti-TIM-1 antibody on TIM-1-Fc fusion protein. Binding curves of the rabbit chimeric version of the anti-TIM-1 antibody 3B3 (NBP2-81119; red line) and isotype control (NBP2-52638, anti-Fluorescein; blue line) to an ELISA plate coated with mouse TIM-1-Fc fusion protein at a concentration of 5 ug/ml. A 3-fold serial dilution from 10000 to 0.17 ng/ml was performed using NBP2-81119. For signal detection, a 1:4000 dilution of anti-rabbit IgG1 HRP antibody was used.")
![Surface Plasmon Resonance: TIM-1/KIM-1/HAVCR Antibody (3B3) [NBP2-81119] - Surface plasmon resonance (SPR) sensorgram TIM-1/KIM-1/HAVCR Antibody (3B3) [NBP2-81119]. 3B3 binding to TIM-1 Fc-fusion protein. Binding of mouse TIM-1 Fc-fusion protein to immbolised the rabbit IgG chimeric version of 3B3 [NBP2-81119]. TIM-1 Fc-fusion protein was injected and allowed to associate with NBP2-81119 between 1080s and 1250s, before a dissociation phase between 1250s and 1800s. TIM-1 Fc-fusion protein showed rapid binding to NBP2-81119 and following ligand removal remained strongly bound. This suggests that NBP2-81119 has a very high affinity for TIM-1 Fc-fusion protein.](http://images.novusbio.com/fullsize/TIM-1-KIM-1-HAVCR-Antibody-3B3-Surface-Plasmon-Resonance-NBP2-81119-img0002.jpg)